Taking your test

Taking this test is a quick and easy way to screen for a vitamin D deficiency but to obtain an accurate result, it’s essential that you do it correctly. Watch our video here for clear, step-by-step instructions on how to take the test. It will show you how to take a sample of your blood and make sure you get the right amount, as well as how long you’ll need to wait for a reliable result. We recommend watching the full video before you start, to ensure you’re ready to collect your sample. You can then use your SPACEBAR to pause the video where needed as you follow along with the step-by-step instructions.

Understanding your lateral flow test results

Understanding your Vitamin D Rapid Test results

To help you understand what steps to take once you have received your results, we’ve put together this page to help you interpret what your results may mean and what you may want to do with these results. Remember: you should always consult a medical professional before making changes to your diet and lifestyle.

Before you continue its important that you have:

  • Read the ‘instructions for use’ leaflet included in your kit
  • Taken the test according to instructions
  • You have interpreted your results against the colour card provided
Between 10 and 20 minutes

Interpreting your results

Once you have collected your sample and performed your test, you must wait 10 minutes after applying your sample into the test cassette, before you read the results. From there, it’s essential that you compare the test (T) line , intensity with the “vitamin D colour card” provided with the kit to get an indication of the vitamin D level in your blood. Do not interpret the result after 20 minutes.

If your test result shows two distinct coloured lines it means your vitamin D level is deficient (Ie. 0-25 nmol/L). This indicates that you may have problems regulating the amount of calcium and phosphate in the body which are two minerals important in maintaining good bone health.

What next?

It is recommended you consider taking a supplement (¹, ³). There are lots of different types of vitamin D supplement and your pharmacist will be able to recommend the best one for you.

During the summer and spring months, most people can make enough vitamin D by going out for short periods in the sun providing they have some skin exposed (e.g. forearms and lower legs). This might not be possible for some people, and you should always practice safe sun exposure, taking care not to burn in the sun.

To confirm your result, or to get an exact measurement of the level of vitamin D in your blood, you might want to consider taking a confirmatory blood test. This type of test is sent off to a laboratory for analysis and can give an accurate reading of your vitamin D levels. You can then take your results to a healthcare professional who will give you advice on what to do next.

When both coloured lines appear, but the ‘control’ (C) line is darker, it means your vitamin levels are insufficient (ie. 25-75 nmol/L).

What next?

Small changes to your lifestyle may help improve your vitamin D insufficiency.

It is recommended that if you are not already taking a vitamin D supplement, you should consider starting one. There are lots of different types of vitamin D supplement and your pharmacist will be able to recommend the best one for you.

During the summer and spring months, most people can make enough vitamin D by going out for short periods in the sun providing they have some skin exposed (e.g. forearms and lower legs). This might not be possible for some people, and you should always practice safe sun exposure, taking care not to burn in the sun.

You may want to consider taking a follow up test in 6 months’ time to monitor whether there has been any improvement in your vitamin D levels.

To confirm your result, or to get an exact measurement of the level of vitamin D in your blood, you might want to consider taking a confirmatory blood test. This type of test is sent off to a laboratory for analysis and can give an accurate reading of your vitamin D levels. You can then take your results to a healthcare professional who will give you advice on what to do next.

If your test results shows two lines, but only the colour in the ‘test’ (T) line is faint, it indicates your vitamin D levels is in the normal range (75-250 nmol/L), this is considered a marker of good health.

What next?

A sufficient level of vitamin D suggests your diet and lifestyle is at an optimum level for this vitamin. This may be because you are getting adequate exposure to sunlight or a supplement you are taking suits you well. Moving forward, ensure you’re practising safe sun exposure and speak to your pharmacist if you are considering changing your supplementation.

You may want to consider taking a follow up test in 6 months’ time to monitor your levels of vitamin D for any changes.

To confirm your result, or to get an exact measurement of the level of vitamin D in your blood, you might want to consider taking a confirmatory blood test. This type of test is sent off to a laboratory for analysis and can give an accurate reading of your vitamin D levels.

If only one coloured line appears in the control (C) line it indicates you have high levels of vitamin D. Extremely high levels of vitamin D, where levels are over 200nmol/L, can cause vitamin D toxicity (², ³). This can result in the body absorbing too much calcium.

What next?

Vitamin D toxicity is almost always caused by taking too many supplements (², ³). If you’re levels are too high, you should reduce the amount of supplement you’re taking in discussion with your doctor or a pharmacist.

You may want to consider taking a follow up test in 6 months’ time to monitor whether there has been any improvement in your vitamin D levels.

To confirm your result, or to get an exact measurement of the level of vitamin D in your blood, you might want to consider taking a confirmatory blood test. This type of test is sent off to a laboratory for analysis and can give an accurate reading of your vitamin D levels. You can then take your results to a healthcare professional who will give you advice on what to do next.

If a control (C) line fails to appear, it is likely there was an insufficient volume of specimen to test, or the incorrect testing procedure was followed.

What next?

We recommend that you take another test. If this problem persists, please contact our customer care team for further assistance.

References: 1: Holick MF, Chen TC. Vitamin D deficiency: a worldwide problem with health consequences. Am J Clin Nutr. 2008 Apr;87(4):1080S-6S. 2: Lips P, van Schoor NM. The effect of vitamin D on bone and osteoporosis. Best Pract Res Clin Endocrinol Metab. 2011 Aug;25(4):585–91. 3: Overview | Vitamin D: supplement use in specific population groups | Guidance | NICE [Internet]. NICE; [cited 2022 Aug 15]. Available from: Overview | Vitamin D: supplement use in specific population groups | Guidance | NICE

Lifestyle

Did you know?

Did you know that your risk of developing certain vitamin and mineral deficiencies can be identified by looking at your DNA?

Our team of scientists and experts have selected the latest genetic markers relating to vitamin and mineral absorption and metabolism to help you make small changes now that can reduce the risk of deficiencies later.